Journal: BMC Immunology

Article Title: Prostaglandin E2 stimulates COX-2 expression via mitogen-activated protein kinase p38 but not ERK in human follicular dendritic cell-like cells

doi: 10.1186/s12865-020-00347-y

Figure Lengend Snippet: p38 MAPK is necessary for the PGE 2 -induced COX-2 expression in FDC-like cells. a The effects of ERK and p38 knockdown on COX-2 induction by PGE 2 were examined with FDC-like cells that had been transfected with control or siRNA against ERK or p38 before the addition of PGE 2 (1 μM). b The effects of PD098059 (PD, 50 μM) and SB203580 (SB, 10 μM) on COX-2 induction by PGE 2 were examined. FDC-like cells (1 × 10 5 cells) were cultured in the presence of PD098059 or SB203580 for 30 min and then added with PGE 2 (1 μM) for 8 h. The expression levels of COX-1, COX-2, and β-actin were measured by immunoblotting. Representative immunoblots and statistical analysis data (mean ± SEM) of three independent experiments are shown. An asterisk(s) indicates a significant difference (*, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, non-significant). v, vehicle

Article Snippet: Specific antibodies to COX-1 and COX-2 were purchased from Cell Signaling Technology.

Techniques: Expressing, Transfection, Cell Culture, Western Blot

Journal: BMC Immunology

Article Title: Prostaglandin E2 stimulates COX-2 expression via mitogen-activated protein kinase p38 but not ERK in human follicular dendritic cell-like cells

doi: 10.1186/s12865-020-00347-y

Figure Lengend Snippet: IL-1β-stimulated COX-2 expression in FDC-like cells depends on p38 MAPK. a The effects of ERK and p38 knockdown on COX-2 induction by IL-1β were examined with FDC-like cells that had been transfected with control or siRNA against ERK or p38 before the addition of IL-1β (10 pg/ml). The expression levels of COX-1, COX-2, and β-actin were measured by immunoblotting. b The amounts of 6-keto-PGF 1α in culture supernatants of (A) experiments were measured by EIA as described in Methods . c The effects of PD098059 (PD, 50 μM) and SB203580 (SB, 10 μM) on COX-2 induction by IL-1β were examined. Representative immunoblots and statistical analysis data (mean ± SEM) of three independent experiments are shown. An asterisk(s) indicates a significant difference (*, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, non-significant). v, vehicle

Article Snippet: Specific antibodies to COX-1 and COX-2 were purchased from Cell Signaling Technology.

Techniques: Expressing, Transfection, Western Blot

Journal: BMC Immunology

Article Title: Prostaglandin E2 stimulates COX-2 expression via mitogen-activated protein kinase p38 but not ERK in human follicular dendritic cell-like cells

doi: 10.1186/s12865-020-00347-y

Figure Lengend Snippet: Proposed model for the central role of p38 mitogen-activated protein kinase in COX-2 expression in human FDC. The paracrine or autocrine effects of PGE 2 are exerted to FDC via EP2 and EP4 receptors. PGE 2 promotes COX-2 expression by stimulating the activity of p38 activity and repressing that of Akt. PGs released from FDC take part in the GC reactions in a paracrine fashion to regulate important functions of lymphocytes. For example, PGE 2 enhances the survival and antigen-presenting capability of GC B cells

Article Snippet: Specific antibodies to COX-1 and COX-2 were purchased from Cell Signaling Technology.

Techniques: Expressing, Activity Assay

Journal: Biomedicines

Article Title: Effect of Serping1 siRNA Injection on Dopaminergic Cell Reduction in an MPTP-Induced Parkinson’s Disease Mouse Model

doi: 10.3390/biomedicines14030569

Figure Lengend Snippet: Changes in cyclooxygenase-2 (COX2) and inducible nitric oxide synthase (iNOS) levels in the striatum by injecting Serping1 siRNA. ( A ) Immunoblot results of COX2 and iNOS in the striatum of C, NC, SER1, and NAC groups. ( B ) The immunoblot of COX2 and iNOS is shown in a graph. COX2 and iNOS levels increased significantly in the NC group. However, COX2 levels decreased significantly in the SER1 and NAC groups, and iNOS levels also decreased in the SER1 and NAC groups; (COX2, n = 3, F(3,8) = 14.270, ANOVA = 0.001; iNOS, n = 3, F(3,8) = 4.366, ANOVA = 0.042) means ± standard errors, * p < 0.05, ** p < 0.005, # p = 0.067.

Article Snippet: Then, the membranes were incubated with primary antibodies: anti-TH (1:2000; Santa Cruz Biotechnology, Dallas, TX, USA), anti- Serping1 (1:2000; Cloud clone Corp., Katy, TX, USA), anti-β-actin (1:5000; Santa Cruz Biotechnology), anti-pSer129-α-syn (1:1000; FUJIFILM Wako Pure Chemical Corp., Osaka, Japan), anti-cyclooxygenase-2 (COX2) (1:2000; Proteintech group, Rosemont, IL, USA), and anti-nitric oxide synthase (iNOS) (1:2000; Calbiochem, Darmstadt, Germany).

Techniques: Western Blot

Journal: iScience

Article Title: LETMD1 regulates mitochondrial protein synthesis and import to guard brown fat mitochondrial integrity and function

doi: 10.1016/j.isci.2024.110944

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal anti-MTCO2 , Proteintech , Cat #: 55070-1-AP, RRID: AB_10859832.

Techniques: Recombinant, Modification, Saline, Reverse Transcription, Bicinchoninic Acid Protein Assay, Western Blot, Software